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cas9 nickase cas9d10a  (Addgene inc)


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    Addgene inc cas9 nickase cas9d10a
    Cas9 Nickase Cas9d10a, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cas9+nickase+cas9d10a/pCas9D10A_GFP+(Plasmid+%2344720)/pm41839426-180-4-7
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    CRISPR:

    Article Title: High-throughput screening of ALS patient iPSC-derived spinal motor neurons identifies novel compounds that increase neurofilament light chain expression.
    Article Snippet: .. CRISPR guide RNA pairs, Cas9 nickase Cas9D10A (Addgene plasmid #44720), and a donor plasmid were introduced into the iPSCs by electroporation. ..

    Plasmid Preparation:

    Article Title: High-throughput screening of ALS patient iPSC-derived spinal motor neurons identifies novel compounds that increase neurofilament light chain expression.
    Article Snippet: .. CRISPR guide RNA pairs, Cas9 nickase Cas9D10A (Addgene plasmid #44720), and a donor plasmid were introduced into the iPSCs by electroporation. ..

    Electroporation:

    Article Title: High-throughput screening of ALS patient iPSC-derived spinal motor neurons identifies novel compounds that increase neurofilament light chain expression.
    Article Snippet: .. CRISPR guide RNA pairs, Cas9 nickase Cas9D10A (Addgene plasmid #44720), and a donor plasmid were introduced into the iPSCs by electroporation. ..



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    Image Search Results


    Journal: Cell Reports

    Article Title: The glucocorticoid receptor recruits the COMPASS complex to regulate inflammatory transcription at macrophage enhancers

    doi: 10.1016/j.celrep.2021.108742

    Figure Lengend Snippet:

    Article Snippet: For mutation of RAW264.7 cells, pU6.chimeric.gRNA , pCAG.Cas9D10A-EGFP (expressing Cas9 nickase, a gift from I. Burtscher) and a single strand DNA repair template (IDT, see )) were electroporated using the NEON electroporation system, FACS sorted after 24h and seeded as single clones.

    Techniques: Recombinant, Protease Inhibitor, SYBR Green Assay, RNA Extraction, Purification, Transfection, Software